The aim of this seminar is to provide an introduction to continuous chromatography with hands-on practice with capture and polishing processes for biomolecules. These processes lead to improvements in productivity and manufacturing costs and may be even enabling in difficult purification challenges, such as antibody-drug conjugates or biosimilars. Attendees will acquire the basic tools to design, run and evaluate multicolumn processes and to quantify these improvements, serving as basis for an economic evaluation. As the least complex of all multi-column processes, the workshop is focused on twin column chromatography.
Introduction to continuous chromatography for biomolecules
Theory of multi-column chromatography
Design of multi-column chromatography processes
Hands-on training on twin column equipment capture and polishing applications
Process performance evaluation and scale-up
This workshop does not cover 4-zone SMB, chiral and small molecule separations.
Content
Lecture 1: General introduction
Reception
Lecture 2: Basics of continuous chromatography
Lab workshop 1: Batch capture
Lecture 3: Insight into continuous capture processes
Lab workshop 2: Continuous Capture
Lecture 4: Performance evaluation of continuous chromatography
Evaluation workshop 1 and 2: Batch and Continuous Capture
Lab workshop 3: Gradient Batch
Presentation of BT, Batch and CSMB results by groups
Lecture 5: Continuous polishing of biomolecules
Evening program
Evaluation Workshop 3: Gradient Batch
Lab workshop 4: MCSGP
Lecture 6: Integrated continuous chromatography
Lecture 7: Modelling and simulations
Simulation Workshop: Process simulation
Lecture 8: N-Rich process for impurity isolation
Lecture 9: Continuous frontal and flow-through processing
Evaluation Workshop 4: MCSGP process
Presentation of MCSGP results by groups
Lecture 10: Scale-up of continuous chromatography
Lecture 11: Course wrap up
Overview
This seminar is aimed at industry and academic separation scientists and bioprocess development engineers who already have some familiarity with single column chromatography and who want to broaden their understanding of chromatographic processes and look at new and more efficient ways to separate and polish biomolecules.
The seminar takes the form of presentations and interactive workshops using laboratory-scale Contichrom CUBE twin column separation & purification systems. Supervisors and graduate assistants will support the participants during the interactive workshops and data analysis sessions.
Note: As the workshops will take place in a laboratory environment we ask that participants dress appropriately. Safety glasses and lab coats will be provided.
Number of participants: A minimum of 8 and a maximum of 12 participants will be accepted in the seminar.
The seminar fee is CHF 4'000. This includes lecture summaries in paper and electronic formats, materials used during the workshop, internet access (wifi), lunch, coffee breaks and evening programs. It does not include accommodation, travel costs or catering other than indicated above.
Massimo Morbidelli Ph.D., Professor of Chemical Reaction and Separation Technologies in the Department of Chemistry, Politecnico di Milano. A pioneer in preparative continuous chromatography and in particular in the application of multicolumn technologies for protein purification in the pharma industry, Prof. Morbidelli has co-authored over 500 research articles and four books. He serves as associate editor for the Industrial & Engineering Chemical Research journal of the ACS and is the recipient of the 2005 RH Wilhelm award from the AIChE and of the 2014 Gerhard Damkoehler medal of DECHEMA. He is a co-founder of ChromaCon AG in Zurich.
Thomas Müller-Späth, Ph.D., Director of RnD at ChromaCon AG in Zurich. After an assignment at Bayer Health-care, Thomas completed his doctoral work on continuous chromatography of biomolecules in the group of Prof. Morbidelli, and co-founded ChromaCon AG to bring the technology to the market. He has been working on research projects with industrial partners, development of chromatography processes and equipment, and IP management. He has presented on numerous workshops and conferences on continuous chromatography and has co-authored over 20 publications and patents.
Supervisors and tutors: Sebastian Vogg, Richard Weldon, Lars Aumann
CHF 4’000 (including lecture summaries in paper and electronic formats, materials used during the workshop, internet access, lunch, coffee breaks and evening programs)
