A general workflow for tangential flow filtration perfusion scale-up

Patrick Romann, Ken Lee, Venkatesh Natarajan, Daniel Ley, Claes Nymand Nilsson, David Garcia-Munzer, Dominik Schieman, Mahbod Hajighasemi, Alex Zaytsev, Jason Friedman, Loic Chappuis, Tobias Habicher, Colin Jaques, Alex Cohen, Matthew Gagnon, Edward Chan, Daniel Karst, Philip Giller, Antony Sibilia, Andrew L. Zydney and Thomas K. Villiger, Biotechnology and Bioengineering

Synthetic spectral libraries for Raman model calibration

Louis V. Hellequin, Vicent J. Borràs, Patrick Romann, Nandita Vishwanathan, Jonathan Souquet and Thomas K. Villiger, Analytical and Bioanalytical Chemistry

Enrichment of full AAV2 using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP)

Julia M. Müller, Tereza Müllerová, Daniela Tobler, Damian Hauri, Richard Plieninger, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg, Thomas Müller-Späth and Thomas K. Villiger, Biotechnology and Bioengineering

Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics

Sherin Panikulam, Hannah Morgan, MichaelGutknecht, Thomas K. Villiger, Nicolas Lebesgue and Anette Karle, Biotechnology Advances

Assessing the adjuvant potential of CHO host cell proteins using an in vitro dendritic cell assay.

Sherin Panikulam, Hannah Morgan, Michael Gutknecht, Anette Karle, Atchaya Rajaratnam, Jennifer Muntwyler, Oliver Anderka, Nicolas Lebesgue and Thomas K. Villiger, Biotechnology and Bioengineering

Experimental determination of maximum shear stress in Mobius® Breez perfusion microbioreactors and comparative analysis with stirred tank bioreactors

Patrick Romann, Dan Trunov, Ondřej Šrom, Harry L.T. Lee, Kevin S. Lee, Ryan Trocki , David Ephraim, Jean-Marc Bielser, Jonathan Souquet, Miroslav Šoóš, Thomas K. Villiger, Biochemical Engineering Journal

Host cell protein networks as a novel co-elution mechanism during protein A chromatography

Sherin Panikulam, Alexander Hanke, Frieder Kroener, Anette Karle, Oliver Anderka, Thomas K. Villiger, Nicolas Lebesgue, Biotechnology and Bioengineering

Maduramycin, a novel glycosylation modulator for mammalian fed-batch and steady-state perfusion processes

Patrick Romann, Thomas Vuillemin, Silvia Pavone, Martin Jordan, Arnaud Perilleux, Jonathan Souquet, Jean‐Marc Bielser, Christoph Herwig, Thomas K. Villiger, Journal of Biotechnology

Raman‐controlled pyruvate feeding to control metabolic activity and product quality in continuous biomanufacturing

Patrick Romann, Sebastian Schneider, Daniela Tobler, Martin Jordan, Arnaud Perilleux, Jonathan Souquet, Christoph Herwig, Jean‐Marc Bielser, Thomas K. Villiger, Biotechnology Journal

Co-current filtrate flow in TFF perfusion processes: Decoupling transmembrane pressure from crossflow to improve product sieving

Patrick Romann, Philip Giller, Antony Sibilia, Christoph Herwig, Andrew L Zydney, Arnaud Perilleux, Jonathan Souquet, Jean‐Marc Bielser, Thomas K Villiger, Biotechnology and Bioengineering

Maximizing yield of perfusion cell culture processes: evaluation and scale-up of continuous bleed recycling

Patrick Romann, Jakub Kolar, Loïc Chappuis, Christoph Herwig, Thomas K. Villiger, Jean-Marc Bielser, Biochemical Engineering Journal

Advancing Raman model calibration for perfusion bioprocesses using spiked harvest libraries

Patrick Romann, Jakub Kolar, Daniela Tobler, Christoph Herwig, Jean-Marc Bielser, Thomas K. Villiger, Biotechnology Journal

Development of a unique rapid test to detect antibodies directed against an extended RBD of SARS-CoV-2 spike protein

Larissa Brosi, Eric Kübler, Anna Weston, Patrick Romann, Sherin Panikulam, Lorin Dirscherl, Michael Gerspach, Cedric Giegelmann, Daniele Dolce, Marie-Eve Überschlag, Anna Melone, Frank I Bantleon, Thomas K Villiger, Christian-B Gerhold, CHIMIA, Volume 75, Issue 5, Pages 446-452.

Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

Thomas K Villiger, Benjamin Neunstoecklin, Daniel J Karst, Eric Lucas, Matthieu Stettler, Hervé Broly, Massimo Morbidelli, Miroslav Soos, Biochemical Engineering Journal, Volume 131, Pages 84-94.

Proteomic analysis of micro-scale bioreactors as scale-down model for a mAb producing CHO industrial fed-batch platform

Vania Bertrand, Sebastian Vogg, Thomas K Villiger, Matthieu Stettler, Hervé Broly, Miroslav Soos, Massimo Morbidelli, Journal of Biotechnology, Volume 279, Pages 27-36.

Modulation and modeling of monoclonal antibody N‐linked glycosylation in mammalian cell perfusion reactors

Daniel J Karst, Ernesto Scibona, Elisa Serra, Jean‐Marc Bielser, Jonathan Souquet, Matthieu Stettler, Hervé Broly, Miroslav Soos, Massimo Morbidelli, Thomas K Villiger, Biotechnology and Bioengineering, 114, Issue 9, Pages 1978-1990.

Glycosylation flux analysis reveals dynamic changes of intracellular glycosylation flux distribution in Chinese hamster ovary fed-batch cultures

Sandro Hutter, Thomas K Villiger, David Brühlmann, Matthieu Stettler, Hervé Broly, Miroslav Soos, Rudiyanto Gunawan, Metabolic Engineering, Volume 43, Pages 9-20.

Influence of protein/glycan interaction on site-specific glycan heterogeneity

Marie‐Estelle Losfeld, Ernesto Scibona, Chia‐Wei Lin, Thomas K Villiger, Robert Gauss, Massimo Morbidelli, Markus Aebi, The FASEB Journal, Volume 31, Issue 10, Pages 4623-4635.

Modeling of the Degradation of Poly(ethylene glycol)-co-(lactic acid)-dimethacrylate Hydrogels

Vincent EG Diederich, Thomas K Villiger, Giuseppe Storti, Marco Lattuada, Macromolecules, Volume 50, Issue 14, Pages 5527-5538.

Controlling the time evolution of mAb N-linked glycosylation – Part II: Model-based predictions

Thomas K Villiger, Ernesto Scibona, Matthieu Stettler, Hervé Broly, Massimo Morbidelli, Miroslav Soos, Biotechnology Progress, Volume 32, Issue 5, Pages 1135-1148.

Controlling the time evolution of mAb N-linked glycosylation – Part I: Microbioreactor experiments

Thomas K Villiger, Anaïs Roulet, Arnaud Périlleux, Matthieu Stettler, Hervé Broly, Massimo Morbidelli, Miroslav Soos, Volume 32, Issue 5, Pages 1123-1134.

Characterization and comparison of ATF and TFF in stirred bioreactors for continuous mammalian cell culture processes

Daniel J Karst, Elisa Serra, Thomas K Villiger, Miroslav Soos, Massimo Morbidelli, Biochemical Engineering Journal, Volume 110, Pages 17-26.

High-throughput profiling of nucleotides and nucleotide sugars to evaluate their impact on antibody N-glycosylation

Thomas K Villiger, Robert F Steinhoff, Marija Ivarsson, Thomas Solacroup, Matthieu Stettler, Hervé Broly, Jasmin Krismer, Martin Pabst, Renato Zenobi, Massimo Morbidelli, Miroslav Soos, Journal of Biotechnology, Volume 229, Pages 3-12.

Pilot-scale verification of maximum tolerable hydrodynamicstress for mammalian cell culture

Benjamin Neunstoecklin, Thomas K Villiger, Eric Lucas, Matthieu Stettler, Hervé Broly, Massimo Morbidelli, Miroslav Soos, Applied Microbiology and Biotechnology, Volume 100, Issue 8, Pages 3489-3498.

Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi

Ivan Hang, Chia-wei Lin, Oliver C Grant, Susanna Fleurkens, Thomas K Villiger, Miroslav Soos, Massimo Morbidelli, Robert J Woods, Robert Gauss, Markus Aebi, Glycobiology, Volume 25, Issue 12, Pages 1335-1349.

Experimental determination of maximum effective hydrodynamic stress in multiphase flow using shear sensitive aggregates

Thomas K Villiger, Massimo Morbidelli, Miroslav Soos, AIChE Journal, Volume 61, Issue 5, Pages 1735-1744.

High-throughput nucleoside phosphate monitoring in mammalian cell fed-batch cultivation using quantitative matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Robert F Steinhoff, Marija Ivarsson, Tobias Habicher, Thomas K Villiger, Jens Boertz, Jasmin Krismer, Stephan R Fagerer, Miroslav Soos, Massimo Morbidelli, Martin Pabst, Renato Zenobi, Biotechnology Journal, Volume 10, Issue 1, Page 190-198.

Evaluating the impact of cell culture process parameters on monoclonal antibody N-glycosylation

Marija Ivarsson, Thomas K Villiger, Massimo Morbidelli, Miroslav Soos, Journal of Biotechnology, Volume 188, Pages 88-96.

Adaptation for survival: Phenotype and transcriptome response of CHO cells to elevated stress induced by agitation and sparging

Jochen B Sieck, Wolfgang E Budach, Zoltan Suemeghy, Christian Leist, Thomas K Villiger, Massimo Morbidelli, Miroslav Soos, Journal of Biotechnology, Volume 189, Pages 94-103.

Development of a Scale-Down Model of hydrodynamic stress to study the performance of an industrial CHO cell line under simulated production scale bioreactor conditions

Jochen B Sieck, Thekla Cordes, Wolfgang E Budach, Martin H Rhiel, Zoltan Suemeghy, Christian Leist, Thomas K Villiger, Massimo Morbidelli, Miroslav Soos, Journal of Biotechnology, Volume 164, Issue 1, Pages 41-49.