Engineered nanoparticles and interfaces for modulation and monitoring of biological systems.

Nanoparticles precisely engineered at the nanoscale have emerged as novel therapeutic and diagnostic agents due to their unique physicochemical properties (e.g. optical, magnetic, electronic, etc.) that are dictated by their size, shape, composition, surface; and can be customized for the desired biological targets and functions. With applications ranging from imaging and detection probes to nanocarriers for (bio)therapeutics delivery, engineered nanoparticles can give unprecedented information on the chemical and physical processes that take place in biological systems, and enable targeted therapeutic interventions.
In the group of BioInterfaces, we aim to address currently unmet biomedical needs in the diagnosis, monitoring, and treatment of disease with engineered nanoparticles tailored for the desired biological targets and functions. We utilize an interdisciplinary approach at the interface between materials chemistry, nanotechnology, and biology for bench-to-bedside development of optical and magnetic detection probes, (pre-)clinical imaging agents, and drug delivery systems based on inorganic and polymeric nanoparticles. We study the diagnostic and therapeutic performance of these nanoparticles in vitro and in vivo using advanced imaging and cell culture techniques and disease models.

Prof.Dr. Oya Tagit, Head of the Group
Anamarija Nikoletc, PhD candidate
Dr. Evangelos Natsaridis, Postdoc
Dr. Bhaskar Gurram, Postdoc
Diana Schweizer, Research Assistant
Maria Camilla Operti, PhD candidate (Radboudumc, Netherlands)

M-SLS-MSc 0223 Molecular and Translational Imaging

M-SLS-F Biomarker

01 Mar 2023: A heartfelt welcome to our new member Anamarija! She is a PhD candidate funded by SNI.

15 Feb 2023: A heartfelt welcome to Evangelos! He will be developing strategies to eliminate antibiotic-resistant infections.

01 Feb 2023: A short piece by Prof.Dr. Tagit on theranostic nanoparticles is published online:

Happy holidays!

01.11.2022: Dr. Bhaskar Gurram joins the BioInterfaces Group as a postdoc. A heartfelt welcome to Bhaskar!

14.09.2022: Prof.Dr. Tagit gives an invited talk at the Biointerfaces International Conference in Zurich

01.09.2022: Our PhD project on ‘Multi-compartment nanofactories for on-site and on-demand drug synthesis and delivery’ is funded by the Swiss Nanoscience Institute!

13.08.2022: A new paper on Translating the Manufacture of Immunotherapeutic PLGA Nanoparticles from Lab to Industrial Scale: Process Transfer and In Vitro Testing published in Pharmaceutics. Many congratulations to Camilla!

01.08.2022: Prof.Dr. Tagit joins the Executive Committee of the Swiss Nanoscience Institute,

01.04.2022: FHNW welcomes the BioInterfaces Group!

Operti, M.C.; Bernhardt, A.; Pots, J.; Sincari, V.; Jager, E.; Grimm, S.; Engel, A.; Benedikt, A.; Hruby, M.; de Vries, J.M.; Figdor, C.G.; Tagit, O.* Translating the Manufacture of Immunotherapeutic PLGA Nanoparticles from Lab to Industrial Scale: Process Transfer and In Vitro Testing. Pharmaceutics 14 1690 (2022)

Operti, M.C.; Bernhardt, A.; Sincari, V.; Jager, E.; Grimm, S.; Engel, A.; Hruby, M.; Figdor, C.G.; Tagit, O*. Industrial Scale Manufacturing and Downstream Processing of PLGA-Based Nanomedicines Suitable for Fully Continuous Operation. Pharmaceutics 14 276 (2022)

Holohan‌‌, C.; ‌‌Hanarahan‌‌, S.; Feely, N.; Li, Peng.; O’Connell, J.; Moss, C.; Carr, M.; Tagit, O.*; Lee, G.U.* Influence of viral transport media and freeze-thaw cycling on the sensitivity of RT-qPCR detection of SARS-CoV-2. Nanoscale (2021)

Operti, M.C.; Bernhardt, A.; Grimm, S.; Engel, A.; Figdor, C.G.; Tagit, O*. PLGA nanoparticles manufacturing: technologies overview and challenges in industrial scale-up. International Journal of Pharmaceutics 605 120807 (2021)

Krekorian, M.; Sandker, G.; Cortenbach, K.R.G.; Tagit, O.; van Riessen, N.K.; Srinivas, M.; Figdor, C.G.; Heskamp, S.; Aarntzen, E.H.J.G. Radiolabelled poly(lactic-co-glycolic acid) nanoparticles for in vivo immune cell imaging. Bioconjugate Chemistry (2021) (Article ASAP DOI: 10.1021/acs.bioconjchem.1c0027)

Verza, B.S.; van den Beucken, J.J.J.P.; Brandt, J.V.; Junior, M.J.; Barão, V.A.R.; Tagit, O.; Spolidorio, D.M.P.; Vergani, C.E.; de Avila, E.D. A long-term controlled drug-delivery with anionic beta cyclodextrin complex in layer-by-layer coating for percutaneous implants devices. Carbohydrate Polymers 257 117604 (2021)

Staal, A.H.J.; Becker, K.; Tagit, O.; van Riessen, N.K.; Koshkina, O.; Veltien, A.; Bouvain, P.; Cortenbach, K.; Scheenen, T.; Flögel, U.; Temme, S.; Srinivas, M. In vivo clearance of 19F MRI imaging nanocarriers is strongly influenced by nanoparticle ultrastructure. Biomaterials 261 120307 (2020)

Dölen, Y.; Valente, M.; Tagit, O.; Jäger, E.; van Dinther, E.A.W.; van Riessen, N.K.; Hruby, M.; Gileadi, U.; Cerundolo, V.; Figdor, C. Nanovaccine administration route is critical to obtain pertinent iNKT cell help for robust anti-tumor T and B cell responses. OncoImmunology 1738813 (2020)

Tagit, O.*, Operti, MC, Dolen, Y, Figdor, CG. Fluorescence imaging to study in vivo accumulation and clearance of drug delivery nanoparticles. European Molecular Imaging Meeting – EMIM 2020

Operti, M.C.; Dölen, Y.; Keulen, J.; van Dinther, E.A.W.; Figdor, C.; Tagit, O.* Microfluidics-assisted size tuning and biological evaluation of PLGA particles. Pharmaceutics 11 590 (2019)

Querol-Cano, L.; Tagit, O.; Dolen, Y.; van Duffelen, A.; Dieltjes, S.; Buschow, S.; Niki, T,; Hirashima, M.; Joosten, B.; van den Dries, K.; Cambi, A.; Figdor, C.; van Spriel, A. Intracellular galectin-9 controls dendritic cell function by maintaining cell membrane rigidity. iScience 22 240-255 (2019)

Ferrari, R.; Martin, G.; Tagit, O.; Cambi, A.; Voituriez, R.; Vassilopoulos, S.; Chavrier, P. Tumor cell invasion in fibrillar collagen I is mediated by MT1-MMP-based force-producing proteolytic contacts. Nature Communications 10 4886 (2019)

de Avila, E.D.; Castro, A.G.B.; Tagit, O.; Krom, B.; Lowik, D.; van Well, A.; Vergani, C.E.; van den Beucken, J.J.J.P. Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components. Applied Surface Science 488 194-204 (2019)

Swider, E.; Maharjan, S.; Houkens, K; Figdor, C.; Srinivas, M., Tagit, O.* Förster resonance energy transfer-based stability assessment of PLGA nanoparticles in vitro and in vivo. ACS Applied Bio Materials 2 1131-1140 (2019)

de Almeida, P.; Jaspers, M.; Vaessen, S.; Tagit, O.; Portale, G.; Rowan, A.E.; Kouwer, P.H.J. Muscle-like stiffening in synthetic hydrogels. Nature Communications 10 609 (2019)

Tagit, O*, Annio, G, Jennings, TL, Hildebrandt, L. Biomarker detection with enhanced sensitivity in FRET-based immunoassays by multiple antibody conjugation on quantum dots. Single-Molecule Sensors and NanoSystems International Conference – S3IC (2019)

Swider, E.; Maharjan, S.; Houkens, K; Figdor, C.; Srinivas, M., Tagit, O.*Förster resonance energy transfer-based stability assessment of polymeric nanoparticles in vitro and in vivo via optical imaging. European Molecular Imaging Meeting – EMIM 2019

Querol-Cano, L.; Tagit, O.; Dolen, Y.; van Duffelen, A.; Dieltjes, S.; Buschow, S.; Niki, T,; Hirashima, M.; Joosten, B.; van den Dries, K.; Cambi, A.; Figdor, C.; van Spriel, A. Intracellular Galectin-9 Controls Dendritic Cell Function by Maintaining Plasma Membrane Rigidity. 2019 SSRN Electronic Journal
DOI: 10.2139/ssrn.3397114

Operti, M.C.; Fecher, D.; van Dinther, E.A.W.; Grimm, S.; Jaber, R.; Figdor, C.G.; Tagit, O*. A comparative assessment of continuous production techniques to generate sub-micron size PLGA particles. International Journal of Pharmaceutics 550 140-148 (2018)

Annio, G.; Jennings, T.L.; Tagit, O*.; Hildebrandt, N.* Sensitivity enhancement of Förster resonance energy transfer immunoassays by multiple antibody conjugation on quantum dots. Bioconjugate Chemistry 29 2082-2089 (2018)

Hildebrandt, N. and Tagit, O.* Colloidal nanoparticles for signal enhancement in optical diagnostic assays. Journal of Nanoscience and Nanotechnology 18 6671-6679 (2018)

Querol-Cano, L.; Tagit, O.; Figdor, C.; van Spriel, A. Galectin-9 is required for dendritic cell function and directly contributes to DC-mediated anti-tumour immunity. 2018 ESMO Open 3 (Suppl 2):A383.1-A383. DOI: 10.1136/esmoopen-2018-EACR25.904

Tagit, O.*; de Ruiter, M.V.; Brasch, M.; Ma, Y.; Cornelissen, J.J.L.M.* Quantum dot encapsulation in virus-like particles with tuneable structural properties and low toxicity. RSC Advances 7 38110-38118 (2017)

Tagit, O.* and Hildebrandt, N.* Fluorescence sensing of circulating diagnostic biomarkers using molecular probes and nanoparticles. ACS Sensors 2 31-45 (2017)

Tagit, O.; Annio, G.; Hildebrandt, N. Terbium to quantum rod Förster resonance energy transfer for homogeneous bioassays with picomolar detection limits. Microchimica Acta 182 1693-1700 (2015)

Gaudin, A.; Yemisci, M.; Eroglu, H.; Lepetre-Mouelhi, S.; Turkoglu, O.F.; Donmez-Demir, B.; Caban, S.; Sargon, M.F.; Garcia-Argote, S.; Pieters, G.; Loreau, O.; Rousseau, B.; Tagit. O.; Hildebrandt, N.; Le Dantec, Y.; Mougin, J.; Valetti, S.; Chacun, H.; Nicolas, V.; Desmaele, D.; Andrieux, K.; Capan, Y.; Dalkara, T.; Couvreur, P. Squalenoyl Adenosine Nanoparticles cross the Blood-Brain Barrier and provide neuroprotection after stroke and neurotrauma. Nature Nanotechnology 9 1054 – 1062 (2014) ERRATUM: Nature Nanotechnology 10 99 (2015)

Gaudin, A.; Tagit, O.; Sobot, D.; Lepetre-Mouelhi, S.; Mougin, J.; Martens, T.F.; Braeckmans, K.; Nicolas, V.; Desmaële, D.; de Smedt, S.C.; Hildebrandt, N.; Couvreur, P.; Andrieux, K. Transport Mechanisms of Squalenoyl-Adenosine Nanoparticles Across the Blood–Brain Barrier. Chemistry of Materials 27 3636-3647 (2015)

Le Guevel. X.; Tagit, O.; Rodríguez, C.E.; Trouillet, V.; Leal, M.P.; Hildebrandt, N. Ligand effect on size, valence state and red/near infrared photoluminescence of bidentate thiol gold nanoclusters. Nanoscale 6 8091-9 (2014)

Tagit, O.; Tomczak, N.; Jafarpour, A.; Janczewski, D.; Han, M-Y.; Vancso, G.J.; Herek, J.L. Influence of the length and grafting density of PNIPAM chains on the colloidal and optical properties of quantum dot/PNIPAM assemblies. Nanotechnology 22 265701 (2011)

Tagit, O.; Janczewski, D.; Tomczak, N.; Han, M-Y.; Herek, J.L.; Vancso, G.J. Nanostructured thermoresponsive quantum dot/PNIPAM assemblies, Eur.Pol. J. 46 1397 – 1403 (2010)

Tomczak, N.; Janczewski, D.; Tagit, O.; Han, M.Y.; Vancso, G.J. Surface Engineering of Quantum Dots with Designer Ligands in Surface Design: Applications in Bioscience and Nanotechnology ISBN-13: 978-3-527-40789-7 – Wiley-VCH, Berlin (2009) (Book chapter)

Tagit, O.; Tomczak, N.; Jańczewski, D.; Benetti, E.M.; Herek, J.L.; Vancso, G.J. Quantum Dot/Stimuli Responsive Polymer Hybrid Platforms. Polym. Mater. Sci. Eng. 101 1251 – 1252 (2009)

Tagit, O.; Tomczak, N.; Janczewski, D.; Benetti, E. M.; Herek, J. L.; & Vancso, G. J. Quantum dot/stimuli responsive polymer hybrid platforms. In Abstracts of Papers of the American Chemical Society (Vol. 238). 1155 16TH ST, NW, Washington, DC 20036 USA: American Chemical Society (2009)

Tagit, O.; Tomczak, N.; Benetti, E.M.; Cesa, Y.; Blum, C.; Subramaniam, V.; Herek, J.L.; Vancso, G.J. Temperature modulated quenching of quantum dots covalently coupled to chain ends of poly (N-isopropyl acrylamide) brushes on gold. Nanotechnology 20 185501 (2009)

Tagit, O.; Tomczak, N.; Vancso, G.J.  Probing the morphology and nanoscale mechanics of single Poly(N-isopropylacrylamide) microgels across the lower-critical-solution temperature by atomic force microscopy. Small 4 119 – 126 (2008)

Featured articles

  • Operti, M.C.; Figdor, C.G., Tagit, O*. Production techniques for the development of sub-micron size polymeric drug delivery formulations evaluated. Featured on Atlas of Science: