Vordiplom (Bachelor) in Biology (University of Münster, 1989)

Diplom (Master) in Biochemistry (University of Witten-Herdecke, 1992)

Promotion (PhD) in Biochemistry Expression, Purification and Characteri-sation of schistosomal Cathepsin B, an immunodiagnostic antigen of Bilharziosis(University of Giessen, 1994)

Scientist at the medical diagnostic company ScheBo Tech (Wettenberg, 1995-97)

Group Leader at the Department of Biochemistry (University of Bayreuth, 1997-2003)

Habilitation in Biochemistry The plasmid pRN1 from the thermoacidophilic archaeon Sulfolobus islandicus (University of Bayreuth, 2004)

Assistant Professor at the Department of Biochemistry (University of Bayreuth, 2004-2007)

Substitute Professor Biochemistry (University of Bayreuth, 2007-2008)

Pubmed link

(1) Lipps, G.; Fullkrug, R.; Beck, E. Cathepsin B of Schistosoma mansoni. Purification and activation of the recombinant proenzyme secreted by Saccharomyces cerevisiae. J.Biol.Chem. 1996, 271, 1717–1725.

(2) Lipps, G. Pankreatische Elastase 1 - Sichere Diagnostik von chronischer und akuter Pankreatitis. Der Klinkarzt 1996, 25, 65–66.

(3) Holz, B.; Dank, N.; Eickhoff, J. E.; Lipps, G.; Krauss, G.; Weinhold, E. Identification of the binding site for the extrahelical target base in N6-adenine DNA methyltransferases by photo-cross-linking with duplex oligodeoxyribonucleotides containing 5-iodouracil at the target position. J.Biol.Chem. 1999, 274, 15066–15072.

(4) Lipps, G.; Krauss, G. Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein. Biochem.J. 1999, 341 (Pt 3), 537–543.

(5) Schweizer, U.; Hey, T.; Lipps, G.; Krauss, G. Photocrosslinking locates a binding site for the large subunit of human replication protein A to the damaged strand of cisplatin-modified DNA. Nucleic Acids Res. 1999, 27, 3183–3189.

(6) Hey, T.; Lipps, G.; Krauss, G. Binding of XPA and RPA to damaged DNA investigated by fluorescence anisotropy. Biochemistry 2001, 40, 2901–2910.

(7) Lipps, G.; Ibanez, P.; Stroessenreuther, T.; Hekimian, K.; Krauss, G. The protein ORF80 from the acidophilic and thermophilic archaeon Sulfolobus islandicus binds highly site-specifically to double-stranded DNA and represents a novel type of basic leucine zipper protein. Nucleic Acids Res. 2001, 29, 4973–4982.

(8) Lipps, G.; Stegert, M.; Krauss, G. Thermostable and site-specific DNA binding of the gene product ORF56 from the Sulfolobus islandicus plasmid pRN1, a putative archael plasmid copy control protein. Nucleic Acids Res. 2001, 29, 904–913.

(9) Hey, T.; Lipps, G.; Sugasawa, K.; Iwai, S.; Hanaoka, F.; Krauss, G. The XPC-HR23B complex displays high affinity and specificity for damaged DNA in a true-equilibrium fluorescence assay. Biochemistry 2002, 41, 6583–6587.

(10) Lipps, G.; Rother, S.; Hart, C.; Krauss, G. A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity. EMBO J. 2003, 22, 2516–2525.

(11) Lipps, G.; Weinzierl, A. O.; von, S. G.; Buchen, C.; Cramer, P. Structure of a bifunctional DNA primase-polymerase. Nat.Struct.Mol.Biol. 2004, 11, 157–162.

(12) Lipps, G. Das Replikationsprotein des archaealen Plasmides pRN1: Funktion, Struktur und Evolution einer neuen DNA Polymerase. Biospektrum 2004, 10, 381–383.

(13) Lipps, G. The replication protein of the Sulfolobus islandicus plasmid pRN1. Biochem.Soc.Trans. 2004, 32, 240–244.

(14) Zeeb, M.; Lipps, G.; Lilie, H.; Balbach, J. Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus. J.Mol.Biol. 2004, 336, 227–240.
(15) Beinoraviciute-Kellner, R.; Lipps, G.; Krauss, G. In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiae. FEBS Lett. 2005, 579, 4535–4540.

(16) Huang, Y.; Krauss, G.; Cottaz, S.; Driguez, H.; Lipps, G. A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus. Biochem.J. 2005, 385, 581–588.

(17) Lysetska, M.; Zettl, H.; Oka, I.; Lipps, G.; Krauss, G.; Krausch, G. Site-specific binding of the 9.5 kilodalton DNA-binding protein ORF80 visualized by atomic force microscopy. Biomacromolecules. 2005, 6, 1252–1257.

(18) Kernchen, U.; Lipps, G. Thermodynamic analysis of the single-stranded DNA binding activity of the archaeal replication protein A (RPA) from Sulfolobus solfataricus. Biochemistry 2006, 45, 594–603.

(19) Lipps, G. Archaeal Plasmids in Archaea. In Evolution, Physiology & Molecular Biology; Garrett, R., Klenk, H. P., Eds.; Blackwell: Oxford, 2006.

(20) Lipps, G. Plasmids and viruses of the thermoacidophilic crenarchaeote Sulfolobus. Extremophiles. 2006, 10, 17–28.

(21) Sollner, S.; Berkner, S.; Lipps, G. Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus. Extremophiles. 2006, 10, 629–634.

(22) Beck, K.; Lipps, G. Properties of an unusual DNA primase from an archaeal plasmid. Nucleic Acids Res. 2007, 35, 5635–5645.

(23) Berkner, S.; Grogan, D.; Albers, S. V.; Lipps, G. Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea. Nucleic Acids Res. 2007, 35, e88.

(24) Berkner, S.; Lipps, G. An active nonautonomous mobile element in Sulfolobus islandicus REN1H1. J.Bacteriol. 2007, 189, 2145–2149.

(25) Berkner, S.; Lipps, G. Characterization of the transcriptional activity of the cryptic plasmid pRN1 from Sulfolobus islandicus REN1H1 and regulation of its replication operon. J.Bacteriol. 2007, 189, 1711–1721.

(26) Berkner, S.; Lipps, G. Genetic tools for Sulfolobus spp.: vectors and first applications. Arch.Microbiol. 2008, 190, 217–230.

(27) Berkner, S.; Lipps, G. Mutation and reversion frequencies of different Sulfolobus species and strains. Extremophiles. 2008, 12, 263–270.

(28) Lipps, G. Archaeal Plasmids. In Plasmids: Current Research and Future Trends; Lipps, G., Ed.; Caister Academic Pres: Norfolk, 2008.

(29) Prato, S.; Vitale, R. M.; Contursi, P.; Lipps, G.; Saviano, M.; Rossi, M.; Bartolucci, S. Molecular modeling and functional characterization of the monomeric primase-polymerase domain from the Sulfolobus solfataricus plasmid pIT3. FEBS J. 2008, 275, 4389–4402.

(30) Lipps, G. Molecular biology of the pRN1 plasmid from Sulfolobus islandicus. Biochem.Soc.Trans. 2009, 37, 42–45.

(31) Sanchez, M.; Drechsler, M.; Stark, H.; Lipps, G. DNA translocation activity of the multifunctional replication protein ORF904 from the archaeal plasmid pRN1. Nucleic Acids Res. 2009, 37, 6831–6848.

(32) Wagner, M.; Berkner, S.; Ajon, M.; Driessen, A. J.; Lipps, G.; Albers, S. V. Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus. Biochem.Soc.Trans. 2009, 37, 97–101.

(33) Weininger, U.; Zeeb, M.; Neumann, P.; Low, C.; Stubbs, M. T.; Lipps, G.; Balbach, J. Structure-based stability analysis of an extremely stable dimeric DNA binding protein from Sulfolobus islandicus. Biochemistry 2009, 48, 10030–10037.

(34) Beck, K.; Vannini, A.; Cramer, P.; Lipps, G. The archaeo-eukaryotic primase of plasmid pRN1 requires a helix bundle domain for faithful primer synthesis. Nucleic Acids Res. 2010, 38, 6707–6718.

(35) Berkner, S.; Wlodkowski, A.; Albers, S. V.; Lipps, G. Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius. Extremophiles. 2010, 14, 249–259.

(36) Detsch, R.; Dieser, I.; Deisinger, U.; Uhl, F.; Hamisch, S.; Ziegler, G.; Lipps, G. Biofunctionalization of dispense-plotted hydroxyapatite scaffolds with peptides: quantification and cellular response. J.Biomed.Mater.Res.A 2010, 92, 493–503.

(37) Lipps, G. Structure and function of the primase domain of the replication protein from the archaeal plasmid pRN1. Biochem.Soc.Trans. 2011, 39, 104–106.

(38) Kufner, K.; Lipps, G. Construction of a chimeric thermoacidophilic beta-endoglucanase. BMC.Biochem. 2013, 14, 11.

(39) Berkner, S.; Hinojosa, M. P.; Prangishvili, D.; Lipps, G. Identification of the minimal replicon and the origin of replication of the crenarchaeal plasmid pRN1. Microbiologyopen. 2014, 3, 688–701.

(40) Boudet, J.; Devillier, J. C.; Allain, F. H.; Lipps, G. Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What’s next? Comput.Struct.Biotechnol.J. 2015, 13, 339–351.

(41) Yanez-Mo, M.; Siljander, P. R.; Andreu, Z.; Zavec, A. B.; Borras, F. E.; Buzas, E. I.; Buzas, K.; Casal, E.; Cappello, F.; Carvalho, J.; et al. Biological properties of extracellular vesicles and their physiological functions. J.Extracell.Vesicles. 2015, 4, 27066.

(42) Fais, S.; O’Driscoll, L.; Borras, F. E.; Buzas, E.; Camussi, G.; Cappello, F.; Carvalho, J.; Cordeiro da, S. A.; Del, P. H.; El, A. S.; et al. Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine. ACS Nano. 2016, 10, 3886–3899.

(43) Puorger, C.; Di Girolamo, S.; Lipps, G. Elucidation of the Recognition Sequence of Sortase B from Bacillus anthracis by Using a Newly Developed Liquid Chromatography-Mass Spectrometry-Based Method. Biochemistry 2017, 56, 2641-2650.

(44) Boudet, J.; Devillier, J.-C.; Wiegand, T.; Salmon, L.; Meier, B.H.; Lipps, G.*; Allain, F.H.T.* A small helical bundle prepares primer synthesis by binding two ATP nucleotides that enhance sequence-specific recognition of the DNA template. Cell 2018, 276, 154-166.e13. * joint senior authors

(45) Di Girolamo, S., Puorger, C., Castiglione, M., Vogel, M., Gébleux, R., Briendl, M., Hell, T., Beerli, R.R., Grawunder, U., Lipps, G., 2019. Characterization of the housekeeping sortase from the human pathogen Propionibacterium acnes: first investigation of a class F sortase. Biochemical Journal 2019, 476, 665–682.Pubmed link

07/00 - 07/07 DFG grant, priority programme „Interface between material and biosystem“: Influence of a nanoporous surface and of biofunctionalization upon biocompatibility towards bone cells, together with Prof. Dr. G. Ziegler (FBI, Bayreuth)

02/01 - 03/04 Grant by Bavarian high-tech initiative: Production of thermostable and acidophilic enzymes for biotechnological purposes, together with Prof. G. Krauss (Department of Biochemistry, Bayreuth)

03/03 - 03/07 DFG grant, priority programme “Genome function and gene regulation in archaea”: In vivo study of gene regulation and gene function in Sulfolobus ssp.: application and development of transformation vectors

02/04 - 01/07 DFG grant: Structure and function of the gene products ORF8O and ORF904 from the plasmid pRN1 of the thermoacidophilic archaeon Sulfolobus islandicus

06/05 - 05/08 DFG grant: Structure and function of the multifunctional replication protein from the plasmid pRN1 from Sulfolobus islandicus

10/06 - 09/09 DFG grant: Study of an extremophilic β-endoglucanase

04/07 - 03/10 DFG grant: Molecular mechanisms of archaeal DNA replication

04/12 - 12/15 SNF grant: Structure and Function of a Primase, together with Prof. Dr. F. Allain (ETHZ)

12/12 – 09/15 CTI grant: Identification of pharmacologic active compounds in the culture supernatant of a commensale E. coli, a commercial production, together with Laves AG

03/14 – 12/17 CTI grant: Development of novel bacterial sortase enzymes for site-specific conjugation of payloads to antibodies, together with NBE Therapeutics

01/16 – 12/18 SNF grant: Structure and Function of a Primase (prolongation), together with Prof. Dr. F. Allain (ETHZ)

01/16 – 06/17 SNI grant: IgG Aptamer-Nanopartikel für die Entwicklung von Zelllinien für die Antikörperproduktion, together with Dr. Martin Held (ETHZ) and FGen

01/18 – 12/19 SNI grant: Efficient capturing of mRNA for single-cell transcriptomics, together with Dr. Martin Held (ETHZ) and Memo Therapeutics

03/19 – 02/21 SNF Bridge Discovery: Efficient Colonic Delivery, together with Prof. Dr. Georgios Imanidis (FHNW)

04/19 – 03/23 SNF: Structural and Functional Characterisation of the Primer Synthesis of Archaeoeukaryotic Primases, together with Prof. Dr. F. Allain (ETHZ)

• Editor for Plasmid, Member of the Editorial Board Extremophiles, Reviewer for Journals (NAR, Mol Microbiol, JMB, AEM, Biochemistry, etc.) and Funding Organisations (Innosuisse, DFG, ANR)